Journal article
Cell-wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties
- Abstract:
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The Arabidopsis sensitive-to-freezing8 (sfr8) mutant exhibits reduced cell wall (CW) fucose levels and compromised freezing tolerance. To examine whether CW fucosylation also affects the response to desiccation, we tested the effect of leaf excision in sfr8 and the allelic mutant mur1-1. Leaf water loss was strikingly higher than in the wild type in these, but not other, fucosylation mutants. We hypothesized that reduced fucosylation in guard cell (GC) walls might limit stomatal closure through altering mechanical properties. Multifrequency atomic force microscopy (AFM) measurements revealed a reduced elastic modulus (Eʹ), representing reduced stiffness, in sfr8 GC walls. Interestingly, however, we discovered a compensatory mechanism whereby a concomitant reduction in the storage modulus (Eʹʹ) maintained a wild-type viscoelastic time response (tau) in sfr8. Stomata in intact leaf discs of sfr8 responded normally to a closure stimulus, abscisic acid, suggesting that the time response may relate more to closure properties than stiffness does. sfr8 stomatal pore complexes were larger than those of the wild type, and GCs lacked a fully developed cuticular ledge, both potential contributors to the greater leaf water loss in sfr8. We present data that indicate that fucosylation-dependent dimerization of the CW pectic domain rhamnogalacturonan-II may be essential for normal cuticular ledge development and leaf water retention.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.0MB, Terms of use)
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(Preview, Supplementary materials, pdf, 709.6KB, Terms of use)
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- Publisher copy:
- 10.1093/jxb/erad039
Authors
- Publisher:
- Oxford University Press
- Journal:
- Journal of Experimental Botany More from this journal
- Volume:
- 74
- Issue:
- 8
- Pages:
- 2680-2691
- Publication date:
- 2023-01-30
- Acceptance date:
- 2023-01-27
- DOI:
- EISSN:
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1460-2431
- ISSN:
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0022-0957
- Language:
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English
- Keywords:
- Pubs id:
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1326287
- Local pid:
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pubs:1326287
- Deposit date:
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2023-01-31
Terms of use
- Copyright holder:
- Panter et al.
- Copyright date:
- 2023
- Rights statement:
- © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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