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Thermal tolerance of Acer campestre (field maple) under heat and drought stress derived from chlorophyll fluorescence

Abstract:

Climate change is increasing the frequency of extreme heat events, making the thermal tolerance of urban trees critical for sustainable city landscapes. We quantified how hydration status influences heat tolerance in $Acer\ campestre$ (field maple) by measuring chlorophyll-fluorescence based dark-adapted thermal tolerance values for the onset ($T_{\mathrm{crit}}$) and 50% reduction ($T_{50}$) of photosystem II efficiency. Measurements were taken at four time points under controlled conditions, with the final measurement including drought stress followed by rehydration of the sample leaves. Linear mixed-effects modelling revealed that treatment significantly affected $T_{\mathrm{crit}}$ ($F_{(2,49)} = 27.6$, $p < 0.001$) but not $T_{50}$ ($F_{(2,49)} = 2.22$, $p = 0.12$). $T_{\mathrm{crit}}$ declined from 41.2--44.5$^\circ$C under well-watered conditions to about 30.4$^\circ$C during drought, then recovered to approximately 44.3$^\circ$C after 24 hours of rehydration. $T_{50}$ remained relatively stable (47--49.9$^\circ$C) across treatments. Principal component and clustering analyses confirmed hydration status as the main driver of variation (PC1 = 80.4% of variance; PERMANOVA $F = 5.47$, $p = 0.001$). A positive correlation between $T_{\mathrm{crit}}$ and $T_{50}$ ($r = 0.61$, $p < 0.01$) indicated coordinated but distinct protective mechanisms operating across stress levels. These findings demonstrate that short-term hydration has a greater influence on photosynthetic heat tolerance than prior drought exposure. $Acer\ campestre$ shows high physiological plasticity, with rapid recovery of $T_{\mathrm{crit}}$ after rehydration, suggesting that maintaining soil moisture through targeted irrigation could significantly enhance tree resilience to increasing urban heat extremes.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/s00468-026-02762-x

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Sub department:
Botnar Institute for Musculoskeletal Sciences
Role:
Author
ORCID:
0000-0002-5922-4178


Publisher:
Springer Nature
Journal:
Trees More from this journal
Volume:
40
Issue:
3
Article number:
66
Publication date:
2026-04-17
Acceptance date:
2026-03-13
DOI:
EISSN:
1432-2285
ISSN:
0931-1890


Language:
English
Keywords:
Pubs id:
2394824
Local pid:
pubs:2394824
Deposit date:
2026-03-25
ARK identifier:

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