Single domain intracellular antibodies: a minimal fragment for direct in vivo selection of antigen-specific intrabodies.

Journal article

There is a major need in target validation and therapeutic applications for molecules that can interfere with protein function inside cells. Intracellular antibodies (intrabodies) can bind to specific targets in cells but isolation of intrabodies is currently difficult. Intrabodies are normally single chain Fv fragments comprising variable domains of the immunoglobulin heavy (VH) and light chains (VL). We now demonstrate that single VH domains have excellent intracellular properties of solubi...

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Authors: Tanaka, T; Lobato, M; Rabbitts, T

• Journal: Journal of molecular biology
• Volume: 331
• Issue: 5
• Pages: 1109-1120
• Publication date: 2003-08-01
• DOI: 10.1016/s0022-2836(03)00836-2
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Antigens; Saccharomyces cerevisiae; Immunoglobulin Fragments; Protein Structure, Tertiary; Cell Transformation, Neoplastic; Base Sequence; COS Cells; Sequence Homology, Amino Acid; Recombinant Proteins; Animals; CHO Cells; Cricetinae; Two-Hybrid System Techniques; 3T3 Cells; Peptide Library; Mice; Plasmids; Amino Acid Sequence; Molecular Sequence Data; Complementarity Determining Regions