Chemically amplified 19F-1H nuclear Overhauser effects.

Journal article

Chemically induced dynamic nuclear polarisation (CIDNP) is explored as a source of nuclear hyperpolarisation in heteronuclear Overhauser effect experiments. A photochemical reaction proceeding through a radical pair intermediate is used to enhance (19)F nuclear magnetisation in 3-fluorotyrosine by more than an order of magnitude with a corresponding increase in the amplitudes of (19)F-(1)H cross-relaxation and cross-correlation effects. The reactions employed are cyclic and leave the sample chemically unchanged. The potential for enhancing the sensitivity of heteronuclear NOEs in (19)F-labelled proteins is discussed.

Authors: Kuprov, I; Hore, P

• Publication status: Published
• Journal: Journal of magnetic resonance (San Diego, Calif. : 1997)
• Volume: 168
• Issue: 1
• Pages: 1-7
• Publication date: 2004-05-01
• DOI: 10.1016/j.jmr.2004.01.011
• EISSN: 1096-0856
• ISSN: 1090-7807
• Language: English
• Keywords: NMR; fluorine; NOE; CIDNP; amino acid