Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair.

Journal article

A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

Authors: Feaver, W; Huang, W; Gileadi, O; Myers, L; Gustafsson, C

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 275
• Issue: 8
• Pages: 5941-5946
• Publication date: 2000-02-01
• DOI: 10.1074/jbc.275.8.5941
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Transcription Factor TFIIH; Ultraviolet Rays; Temperature; Plasmids; Transcription Factors, TFII; Phosphodiesterase Inhibitors; Caffeine; Fungal Proteins; Models, Biological; DNA Repair; Adenosine Triphosphatases; Two-Hybrid System Techniques; Saccharomyces cerevisiae Proteins; DNA Helicases; Genetic Complementation Test; Mutation; TATA-Binding Protein Associated Factors; Transcription Factors; Transcription Factor TFIID