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Journal article

Fluorescence nanoscopy by ground-state depletion and single-molecule return.

Abstract:
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
Publication status:
Published

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Publisher copy:
10.1038/nmeth.1257

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Journal:
Nature methods
Volume:
5
Issue:
11
Pages:
943-945
Publication date:
2008-11-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:0da07fbb-0c1c-4ba1-b408-21e6bce0b664
Source identifiers:
222188
Local pid:
pubs:222188

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