Journal article

Fluorescence nanoscopy by ground-state depletion and single-molecule return.

Abstract:: We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.

Publication status:: Published

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APA Style

Fölling, J., Bossi, M., Bock, H., Medda, R., Wurm, C., Hein, B., Jakobs, S., Eggeling, C., & Hell, S. (2008). Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nature Methods, 5(11), 943–945.

MLA Style

Fölling, J, et al. “Fluorescence Nanoscopy by Ground-State Depletion and Single-Molecule Return.” Nature Methods, vol. 5, no. 11, 2008, pp. 943–45.

Chicago Style

Fölling, J, M Bossi, H Bock, et al. 2008. “Fluorescence Nanoscopy by Ground-State Depletion and Single-Molecule Return.” Nature Methods 5 (11): 943–45.
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Publisher copy:: 10.1038/nmeth.1257

Authors

+ Fölling, J More by this author

Role:: Author

+ Bossi, M More by this author

Role:: Author

+ Bock, H More by this author

Role:: Author

+ Medda, R More by this author

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+ Wurm, C More by this author

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Journal:: Nature methods More from this journal
Volume:: 5
Issue:: 11
Pages:: 943-945
Publication date:: 2008-11-01
DOI:: 10.1038/nmeth.1257
EISSN:: 1548-7105
ISSN:: 1548-7091

Language:: English
Keywords:: Cell Survival

Microscopy, Fluorescence

Spectrometry, Fluorescence

Cell Line

Humans
Pubs id:: pubs:222188
UUID:: uuid:0da07fbb-0c1c-4ba1-b408-21e6bce0b664
Local pid:: pubs:222188
Source identifiers:: 222188
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_0da07fbb0c1c4ba1b40821e6bce0b664

Terms of use

Copyright date:: 2008

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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