Journal article
In vivo two-photon microscopy reveals sensory-evoked serotonin (5-HT) release in adult mammalian neocortex
- Abstract:
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The recent development of genetically encoded fluorescent neurotransmitter biosensors has opened the door to recording serotonin (5-hydroxytryptamine, 5-HT) signaling dynamics with high temporal and spatial resolution in vivo. While this represents a significant step forward for serotonin research, the utility of available 5-HT biosensors remains to be fully established under diverse in vivo conditions. Here, we used two-photon microscopy in awake mice to examine the effectiveness of specific 5-HT biosensors for monitoring 5-HT dynamics in somatosensory cortex. Initial experiments found that whisker stimulation evoked a striking change in 5-HT biosensor signal. However, similar changes were observed in controls expressing green fluorescent protein, suggesting a potential hemodynamic artifact. Subsequent use of a second control fluorophore with emission peaks separated from the 5-HT biosensor revealed a reproducible, stimulus-locked increase in 5-HT signal. Our data highlight the promise of 5-HT biosensors for in vivo application, provided measurements are carried out with appropriate optical controls.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 4.5MB, Terms of use)
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- Publisher copy:
- 10.1021/acschemneuro.3c00725
Authors
- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- 204651/Z/16/Z
- Funder identifier:
- https://ror.org/00k4n6c32
- Grant:
- 953327
- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- MR/T033320/1
- Funder identifier:
- https://ror.org/00cwqg982
- Grant:
- BB/P003796/1
- Publisher:
- American Chemical Society
- Journal:
- ACS Chemical Neuroscience More from this journal
- Volume:
- 15
- Issue:
- 3
- Pages:
- 456-461
- Publication date:
- 2024-01-22
- Acceptance date:
- 2024-01-16
- DOI:
- ISSN:
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1948-7193
- Pmid:
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38251903
- Language:
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English
- Keywords:
- Pubs id:
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1604400
- Local pid:
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pubs:1604400
- Deposit date:
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2024-10-08
- ARK identifier:
Terms of use
- Copyright holder:
- Ocana-Santero et al.
- Copyright date:
- 2024
- Rights statement:
- Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 .
- Notes:
- This work is related to the thesis The role of 5-HT dynamics in cortical population activity throughout mammalian life.
- Licence:
- CC Attribution (CC BY)
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