Journal article

Rehosting of bacterial chaperones for high-quality protein production.

Abstract:: Coproduction of DnaK/DnaJ in Escherichia coli enhances solubility but promotes proteolytic degradation of their substrates, minimizing the yield of unstable polypeptides. Higher eukaryotes have orthologs of DnaK/DnaJ but lack the linked bacterial proteolytic system. By coexpression of DnaK and DnaJ in insect cells with inherently misfolding-prone recombinant proteins, we demonstrate simultaneous improvement of soluble protein yield and quality and proteolytic stability. Thus, undesired side effects of bacterial folding modulators can be avoided by appropriate rehosting in heterologous cell expression systems.

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APA Style

Martínez-Alonso, M., Toledo-Rubio, V., Noad, R., Unzueta, U., Ferrer-Miralles, N., Roy, P., & Villaverde, A. (2009). Rehosting of bacterial chaperones for high-quality protein production. Applied and Environmental Microbiology, 75(24), 7850–7854.

MLA Style

Martínez-Alonso, M, et al. “Rehosting of Bacterial Chaperones for High-Quality Protein Production.” Applied and Environmental Microbiology, vol. 75, no. 24, 2009, pp. 7850–54.

Chicago Style

Martínez-Alonso, M, V Toledo-Rubio, R Noad, et al. 2009. “Rehosting of Bacterial Chaperones for High-Quality Protein Production.” Applied and Environmental Microbiology 75 (24): 7850–54.
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Publisher copy:: 10.1128/aem.01532-09

Authors

+ Martínez-Alonso, M More by this author

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+ Toledo-Rubio, V More by this author

Role:: Author

+ Noad, R More by this author

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+ Unzueta, U More by this author

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+ Ferrer-Miralles, N More by this author

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Journal:: Applied and environmental microbiology More from this journal
Volume:: 75
Issue:: 24
Pages:: 7850-7854
Publication date:: 2009-12-01
DOI:: 10.1128/aem.01532-09
EISSN:: 1098-5336
ISSN:: 0099-2240

Language:: English
Keywords:: Recombinant Proteins

HSP70 Heat-Shock Proteins

Gene Expression

Animals

Protein Folding

Escherichia coli

Spodoptera

HSP40 Heat-Shock Proteins

Escherichia coli Proteins
Pubs id:: pubs:267455
UUID:: uuid:0d6d558b-b7f2-4d3e-83c8-9659902f3ce0
Local pid:: pubs:267455
Source identifiers:: 267455
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_0d6d558bb7f24d3e83c89659902f3ce0

Terms of use

Copyright date:: 2009

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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