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Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection:  Evidence of Two-Step Photolysis.

Abstract:

The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of single-molecule detection (SMD) and for the image quality of scanning confocal microscopy. Concurrent results for the photostability were obtained by two different experimental techniques. First, the photostabilities of several coumarin and rhodamine derivatives in aqueous solution were obtained by monitoring the steady-state fluorescence decay in a quartz cell. Furthermore, an epi-illuminated micr...

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Publication status:
Published

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Publisher copy:
10.1021/ac980027p

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Widengren, J More by this author
Seidel, CA More by this author
Journal:
Analytical chemistry
Volume:
70
Issue:
13
Pages:
2651-2659
Publication date:
1998-07-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:0d62e565-8fbb-4606-909b-62c7474a9718
Source identifiers:
222203
Local pid:
pubs:222203
Language:
English

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