Journal article icon

Journal article

Molecular surveillance identifies multiple transmissions of typhoid in West Africa

Abstract:
The burden of typhoid in sub-Saharan African (SSA) countries has been difficult to estimate, in part, due to suboptimal laboratory diagnostics. However, surveillance blood cultures at two sites in Nigeria have identified typhoid associated with Salmonella enterica serovar Typhi (S. Typhi) as an important cause of bacteremia in children.A total of 128 S. Typhi isolates from these studies in Nigeria were whole-genome sequenced, and the resulting data was used to place these Nigerian isolates into a worldwide context based on their phylogeny and carriage of molecular determinants of antibiotic resistance.Several distinct S. Typhi genotypes were identified in Nigeria that were related to other clusters of S. Typhi isolates from north, west and central regions of Africa. The rapidly expanding S. Typhi clade 4.3.1 (H58) previously associated with multiple antimicrobial resistances in Asia and in east, central and southern Africa, was not detected in this study. However, antimicrobial resistance was common amongst the Nigerian isolates and was associated with several plasmids, including the IncHI1 plasmid commonly associated with S. Typhi.These data indicate that typhoid in Nigeria was established through multiple independent introductions into the country, with evidence of regional spread. MDR typhoid appears to be evolving independently of the haplotype H58 found in other typhoid endemic countries. This study highlights an urgent need for routine surveillance to monitor the epidemiology of typhoid and evolution of antimicrobial resistance within the bacterial population as a means to facilitate public health interventions to reduce the substantial morbidity and mortality of typhoid.
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Publisher copy:
10.1371/journal.pntd.0004781

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Tropical Medicine
Role:
Author

Contributors

Role:
Editor


More from this funder
Funding agency for:
MacLennan, C
Grant:
Clinical Research Fellowship
More from this funder
Funding agency for:
Baker, S
Grant:
100087/Z/12/Z
More from this funder
Funding agency for:
Baker, S
Grant:
100087/Z/12/Z
More from this funder
Funding agency for:
Baker, S
Grant:
100087/Z/12/Z


Publisher:
Public Library of Science
Journal:
PLoS Neglected Tropical Diseases More from this journal
Volume:
10
Issue:
9
Article number:
e0004781
Publication date:
2016-09-22
Acceptance date:
2016-05-25
DOI:
EISSN:
1935-2735
ISSN:
1935-2727
Pmid:
27657909


Language:
English
Keywords:
Pubs id:
pubs:648038
UUID:
uuid:0cf0f515-6c49-47b9-9b86-c346729f913f
Local pid:
pubs:648038
Source identifiers:
648038
Deposit date:
2016-10-24

Terms of use



Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP