CD28 utilizes Vav-1 to enhance TCR-proximal signaling and NF-AT activation.

Journal article

The mechanism through which CD28 costimulation potentiates TCR-driven gene expression is still not clearly defined. Vav-1, an exchange factor for Rho GTPases thought to regulate, mainly through Rac-1, various signaling components leading to cytokine gene expression, is tyrosine phosphorylated upon CD28 engagement. Here, we provide evidence for a key role of Vav-1 in CD28-mediated signaling. Overexpression of Vav-1 in Jurkat cells in combination with CD28 ligation strongly reduced the concentr...

Expand abstract

Authors: Michel, F; Mangino, G; Attal-Bonnefoy, G; Tuosto, L; Alcover, A

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 165
• Issue: 7
• Pages: 3820-3829
• Publication date: 2000-10-01
• DOI: 10.4049/jimmunol.165.7.3820
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Transcription Factors; Receptors, Antigen, T-Cell, alpha-beta; ZAP-70 Protein-Tyrosine Kinase; Cell Cycle Proteins; Adaptor Proteins, Signal Transducing; Phosphorylation; Jurkat Cells; Adjuvants, Immunologic; Antigens, CD28; NFATC Transcription Factors; Phosphoproteins; Humans; Pseudopodia; Transfection; Signal Transduction; Proto-Oncogene Proteins c-vav; Proto-Oncogene Proteins; Protein-Tyrosine Kinases; DNA-Binding Proteins; Nuclear Proteins; src Homology Domains