Digitally adjusting chromogenic dye proportions in brightfield microscopy images.
We present an algorithm to adjust the contrast of individual dyes from colour (red-green-blue) images of dye mixtures. Our technique is based on first decomposing the colour image into individual dye components, then adjusting each of the dye components and finally mixing the individual dyes to generate colour images. Specifically in this paper, we digitally adjust the staining proportions of hematoxylin and eosin (HandE) chromogenic dyes in tissue images. We formulate the physical dye absorp...Expand abstract
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