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Digitally adjusting chromogenic dye proportions in brightfield microscopy images.

Abstract:

We present an algorithm to adjust the contrast of individual dyes from colour (red-green-blue) images of dye mixtures. Our technique is based on first decomposing the colour image into individual dye components, then adjusting each of the dye components and finally mixing the individual dyes to generate colour images. Specifically in this paper, we digitally adjust the staining proportions of hematoxylin and eosin (HandE) chromogenic dyes in tissue images. We formulate the physical dye absorp...

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Author
Journal:
Journal of microscopy
Volume:
245
Issue:
3
Pages:
319-330
Publication date:
2012-03-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Source identifiers:
439032
Language:
English
Keywords:
Pubs id:
pubs:439032
UUID:
uuid:0cb272fd-55e7-4a8b-9556-a62062f091b7
Local pid:
pubs:439032
Deposit date:
2014-02-08

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