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Base-flipping dynamics in a DNA hairpin processing reaction

Abstract:

Many enzymes that repair or modify bases in double-stranded DNA gain access to their substrates by base flipping. Although crystal structures provide stunning snap shots, biochemical approaches addressing the dynamics have proven difficult, particularly in complicated multi-step reactions. Here, we use protein-DNA crosslinking and potassium permanganate reactivity to explore the base-flipping step in tN5 transposition. We present a model to suggest that base flipping is driven by a combinatio...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkm186

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Wellcome Trust More from this funder
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
35
Issue:
8
Pages:
2584-2595
Publication date:
2007-04-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Keywords:
Subjects:
UUID:
uuid:0cae0044-30d3-498c-94b9-d38efc28e5b2
Local pid:
ora:3020
Deposit date:
2009-11-05

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