Journal article
Development of homogeneous luminescence assays for histone demethylase catalysis and binding.
- Abstract:
-
Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technolog...
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- Publication status:
- Published
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- Publisher copy:
- 10.1016/j.ab.2010.04.030
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Authors
Bibliographic Details
- Journal:
- Analytical biochemistry
- Volume:
- 404
- Issue:
- 1
- Pages:
- 86-93
- Publication date:
- 2010-09-01
- DOI:
- EISSN:
-
1096-0309
- ISSN:
-
0003-2697
- Source identifiers:
-
54671
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:54671
- UUID:
-
uuid:0caa9d97-6516-4172-911d-9be883b63dbd
- Local pid:
- pubs:54671
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2010
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