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Development of homogeneous luminescence assays for histone demethylase catalysis and binding.

Abstract:

Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technolog...

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Publication status:
Published

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Publisher copy:
10.1016/j.ab.2010.04.030

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry, BHF Centre of Research Excellence
Role:
Author
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Journal:
Analytical biochemistry
Volume:
404
Issue:
1
Pages:
86-93
Publication date:
2010-09-05
DOI:
EISSN:
1096-0309
ISSN:
0003-2697
URN:
uuid:0caa9d97-6516-4172-911d-9be883b63dbd
Source identifiers:
54671
Local pid:
pubs:54671

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