A simple electroanalytical methodology for the simultaneous determination of dopamine, serotonin and ascorbic acid using an unmodified edge plane pyrolytic graphite electrode.

Journal article

A simple method using an unmodified edge plane pyrolytic graphite electrode (EPPGE) is reported for the simultaneous determination of dopamine (DA), serotonin (ST) and ascorbic acid (AA). The performance of this electrode is superior to other unmodified carbon-based electrodes and also to many modified electrodes in terms of detection limit, sensitivity and peak separation for determination of DA, ST and AA. Using this method, detection limits of 90 nM, 60 nM and 200 nM were obtained for DA, ST and AA respectively. No electrode fouling is observed during a set of experiments and good sensitivity is obtained for the simultaneous determination of DA, ST and AA. The peaks for the three species are well resolved from each other and the electrode is successfully utilised for their determination in standard and real samples.

Authors: Kachoosangi, RT; Compton, R

• Publication status: Published
• Journal: Analytical and bioanalytical chemistry
• Volume: 387
• Issue: 8
• Pages: 2793-2800
• Publication date: 2007-04-01
• DOI: 10.1007/s00216-007-1129-y
• EISSN: 1618-2650
• ISSN: 1618-2642
• Language: English
• Keywords: Serotonin; Hydrogen-Ion Concentration; Ascorbic Acid; Electrodes; Calibration; Dopamine; Graphite; Sensitivity and Specificity