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Kinetic properties of bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from spinach leaves.

Abstract:

A cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Spinacia oleracea leaf library and used to express a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kDa. Soluble protein was purified to homogeneity from S. frugiperda cells infected with recombinant baculov...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
Journal:
European journal of biochemistry / FEBS
Volume:
269
Issue:
4
Pages:
1267-1277
Publication date:
2002-02-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:0c8d4677-0311-4968-ba09-35465960708d
Source identifiers:
32056
Local pid:
pubs:32056

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