Journal article
Sister chromatid cohesion is mediated by individual cohesin complexes
- Abstract:
- Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin's stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclusively monomeric. The monomeric stoichiometry of sororin:cohesin complexes demonstrates that sister chromatid cohesion is conferred by individual cohesin rings, a key prediction of the proposal that cohesion arises from the co-entrapment of sister DNAs.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 4.5MB, Terms of use)
-
- Publisher copy:
- 10.1126/science.adl4606
Authors
+ Wellcome Trust
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- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- 107935/Z/15/Z
+ Cancer Research UK
More from this funder
- Funder identifier:
- https://ror.org/054225q67
- Grant:
- 26747
+ American Association For The Advancement of Science
More from this funder
- Funder identifier:
- https://ror.org/05j0w0e76
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 383
- Issue:
- 6687
- Pages:
- 1122-1130
- Place of publication:
- United States
- Publication date:
- 2024-03-07
- Acceptance date:
- 2024-01-26
- DOI:
- EISSN:
-
1095-9203
- ISSN:
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0036-8075
- Pmid:
-
38452070
- Language:
-
English
- Keywords:
- Pubs id:
-
1804747
- Local pid:
-
pubs:1804747
- Deposit date:
-
2025-12-05
- ARK identifier:
Terms of use
- Copyright holder:
- Ochs et al
- Copyright date:
- 2024
- Rights statement:
- © 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at https://dx.doi.org/10.1126/science.adl4606
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