Journal article
High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo
- Abstract:
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Background. Parvovirus 4 (PARV4) is a recently identified human virus that has been found in livers of patients infected with hepatitis C virus (HCV) and in bone marrow of individuals infected with human immunodeficiency virus (HIV). T cells are important in controlling viruses but may also contribute to disease pathogenesis. The interaction of PARV4 with the cellular immune system has not been described. Consequently, we investigated whether T cell responses to PARV4 could be detected in individuals exposed to blood-borne viruses.
Methods. Interferon γ (INF-γ) enzyme-linked immunospot assay, intracellular cytokine staining, and a tetrameric HLA-A*0201-peptide complex were used to define the lymphocyte populations responding to PARV4 NS peptides in 88 HCV-positive and 13 HIV-positive individuals. Antibody responses were tested using a recently developed PARV4 enzyme-linked immunosorbent assay.
Results. High-frequency T cell responses against multiple PARV4 NS peptides and antibodies were observed in 26% of individuals. Typical responses to the NS pools were > 1000 spot-forming units per million peripheral blood mononuclear cells.
Conclusions. PARV4 infection is common in individuals exposed to blood-borne viruses and elicits strong T cell responses, a feature typically associated with persistent, contained infections such as cytomegalovirus. Persistence of PARV4 viral antigen in tissue from HVC-positive and HIV-positive individuals and/or the associated antiviral T cell response may contribute to disease pathogenesis.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 717.1KB, Terms of use)
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- Publisher copy:
- 10.1093/infdis/jir036
Authors
Contributors
- Role:
- Other
- Role:
- Other
- Publisher:
- Oxford University Press
- Journal:
- Journal of Infectious Diseases More from this journal
- Volume:
- 203
- Issue:
- 10
- Pages:
- 1378-1387
- Publication date:
- 2011-01-01
- Edition:
- Publisher's version
- DOI:
- EISSN:
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1537-6613
- ISSN:
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0022-1899
- Language:
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English
- Keywords:
- Subjects:
- UUID:
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uuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a3
- Local pid:
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ora:6443
- Deposit date:
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2012-08-15
Terms of use
- Copyright holder:
- R Simmons et al
- Copyright date:
- 2011
- Notes:
- This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/byc/3.0), which permits unrestricted nonommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- Other
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