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Tumor protease-activated, pore-forming toxins from a combinatorial library.

Abstract:

We describe a library of two-chain molecular complementation mutants of staphylococcal alpha-hemolysin that features a combinatorial cassette encoding thousands of protease recognition sites in the central pore-forming domain. The cassette is flanked by a peptide extension that inactivates the protein. We screened the library to identify alpha-hemolysins that are highly susceptible to activation by cathepsin B, a protease that is secreted by certain metastatic tumor cells. Toxins obtained by ...

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Publication status:
Published

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Publisher copy:
10.1038/nbt0796-852

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemical Biology
Role:
Author
Journal:
Nature biotechnology More from this journal
Volume:
14
Issue:
7
Pages:
852-856
Publication date:
1996-07-01
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
Language:
English
Keywords:
Pubs id:
pubs:52298
UUID:
uuid:0c775c48-f280-479b-9e50-30f99705d30e
Local pid:
pubs:52298
Source identifiers:
52298
Deposit date:
2013-11-17

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