Interallelic complementation provides genetic evidence for the multimeric organization of the Phycomyces blakesleeanus phytoene dehydrogenase.

Journal article

The Phycomyces blakesleeanus wild-type is yellow, because it accumulates beta-carotene as the main carotenoid. A new carotenoid mutant of this fungus (A486) was isolated, after treatment with ethyl methane sulfonate (EMS), showing a whitish coloration. It accumulates large amounts of phytoene, small quantities of phytofluene, zeta-carotene and neurosporene, in decreasing amounts, and traces of beta-carotene. This phenotype indicates that it carries a leaky mutation affecting the enzyme phytoe...

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Authors: Sanz, C; Alvarez, M; Orejas, M; Velayos, A; Eslava, A

• Journal: European journal of biochemistry / FEBS
• Volume: 269
• Issue: 3
• Pages: 902-908
• Publication date: 2002-02-01
• DOI: 10.1046/j.0014-2956.2001.02724.x
• EISSN: 1432-1033
• ISSN: 0014-2956
• Language: English
• Keywords: Genetic Complementation Test; Amino Acid Sequence; Ethyl Methanesulfonate; Molecular Sequence Data; Cloning, Molecular; Alleles; Fungi; Oxidoreductases; Mutation