Journal article
Composition and antigenic effects of individual glycan sites of a trimeric HIV-1 envelope glycoprotein
- Abstract:
- The HIV-1 envelope glycoprotein trimer is covered by an array of N-linked glycans that shield it from immune surveillance. The high density of glycans on the trimer surface imposes steric constraints limiting the actions of glycan processing enzymes, such that multiple under-processed structures remain on specific areas. These oligomannose glycans are recognized by broadly neutralizing antibodies (bNAbs) that are not thwarted by the glycan shield but, paradoxically, target it. Our site-specific glycosylation analysis of a soluble, recombinant trimer (BG505 SOSIP.664) maps the extremes of simplicity and diversity of glycan processing at individual sites and reveals a mosaic of dense clusters of oligomannose glycans on the outer domain. Although individual sites usually minimally affect the global integrity of the glycan shield, we identify examples of how deleting some glycans can subtly influence neutralization by bNAbs that bind at distant sites. The network of bNAb-targeted glycans should be preserved on vaccine antigens.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Publisher copy:
- 10.1016/j.celrep.2016.02.058
Authors
+ Corpus Christi College, Oxford
More from this funder
- Funding agency for:
- Behrens, A
- Grant:
- Chris Scanlan Memorial Scholarship
+ International AIDS Vaccine Initiative
More from this funder
- Grant:
- International AIDS Vaccine Initiative
+ Scripps Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery
More from this funder
- Grant:
- CHAVI-ID 1UM1AI100663
- Publisher:
- Cell Press
- Journal:
- Cell Reports More from this journal
- Volume:
- 14
- Issue:
- 11
- Pages:
- 2695-2706
- Publication date:
- 2016-03-22
- Acceptance date:
- 2016-02-10
- DOI:
- ISSN:
-
2211-1247
- Pubs id:
-
pubs:607239
- UUID:
-
uuid:0c5027cf-0add-4a9f-b6c9-579618d6963f
- Local pid:
-
pubs:607239
- Source identifiers:
-
607239
- Deposit date:
-
2016-03-02
- ARK identifier:
Terms of use
- Copyright holder:
- Behrens et al
- Copyright date:
- 2016
- Notes:
- © 2016 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
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