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Improved characterisation and quantification of proteins using differential isotope labeling

Abstract:: The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.

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APA Style

Mohammed, S., Cristea, I., Brancia, F., Oliver, S., & Gaskell, S. (2002). Improved characterisation and quantification of proteins using differential isotope labeling. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 391–392.

MLA Style

Mohammed, S., et al. “Improved Characterisation and Quantification of Proteins Using Differential Isotope Labeling.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 2002, pp. 391–92.

Chicago Style

Mohammed, S, I Cristea, F Brancia, S Oliver, and S Gaskell. 2002. “Improved Characterisation and Quantification of Proteins Using Differential Isotope Labeling.” In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 391–92.
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Authors

+ Mohammed, S More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Physical & Theoretical Chem
Role:: Author

+ Cristea, I More by this author

Role:: Author

+ Brancia, F More by this author

Role:: Author

+ Oliver, S More by this author

Role:: Author

+ Gaskell, S More by this author

Role:: Author

Host title:: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages:: 391-392
Publication date:: 2002-01-01

Pubs id:: pubs:385496
UUID:: uuid:0c099df3-f47a-48a3-87fd-6072e70e3fb8
Local pid:: pubs:385496
Source identifiers:: 385496
Deposit date:: 2013-11-16

Terms of use

Copyright date:: 2002

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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