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Improved characterisation and quantification of proteins using differential isotope labeling

Abstract:

The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional b...

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
Pages:
391-392
Publication date:
2002-01-01
URN:
uuid:0c099df3-f47a-48a3-87fd-6072e70e3fb8
Source identifiers:
385496
Local pid:
pubs:385496

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