Journal article icon

Journal article

ATP-sensitive K-channels in HIT T15 beta-cells studied by patch-clamp methods, 86Rb efflux and glibenclamide binding.

Abstract:

ATP-sensitive K-channels in the cloned beta-cell line HIT T15 were studied by patch-clamp methods; by measurement of 86Rb efflux; and by [3H]glibenclamide binding to isolated membrane preparations. In inside-out patches a 50 pS K-channel was found which was blocked by ATP or tolbutamide applied to the intracellular membrane surface. A minimum estimate of about 500 channels per beta-cell was obtained by combining whole-cell and single-channel data. The rate of efflux of 86Rb from 86RbCl-loaded...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1007/bf00373140

Authors


Ashcroft, SJ More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Publisher:
Springer-Verlag
Journal:
Pflugers Archiv : European journal of physiology
Volume:
415
Issue:
1
Pages:
47-55
Publication date:
1989-10-05
DOI:
EISSN:
1432-2013
ISSN:
0031-6768
URN:
uuid:0be2ff29-d959-4f5f-aacf-6d43e32e4fcd
Source identifiers:
113910
Local pid:
pubs:113910

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP