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Establishment of a typing enzyme-linked immunosorbent assay for foot and mouth disease antigen, using reagents against viruses endemic in Thailand.

Abstract:

Antisera were produced at a central laboratory in Thailand against the endemic serotypes (O, A and Asia 1) of foot and mouth disease (FMD) virus. At a regional veterinary laboratory, these antisera were used in an indirect sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and serotyping of FMD virus (FMDV) antigen. ELISA readings of < 0.10 optical density (OD) units were considered negative. This was verified using fifty tissue samples which were known to be negative for...

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Publication status:
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Thailand/Laos MOP
Chamnanpood, C More by this author
Linchongsubongkoch, W More by this author
Nakarungkul, N More by this author
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Journal:
Revue scientifique et technique (International Office of Epizootics)
Volume:
13
Issue:
3
Pages:
701-709
Publication date:
1994-09-05
EISSN:
1608-0637
ISSN:
0253-1933
URN:
uuid:0bc484e3-01ff-4505-a8b1-ca8685420b81
Source identifiers:
94651
Local pid:
pubs:94651

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