Journal article icon

Journal article

A molecular assay for sensitive detection of pathogen-specific T-cells

Abstract:

Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-γ production using real time quantitative PCR (qPCR) for two reporters - monokine-induced by IFN-γ (MIG) and the IFN-γ inducible protein-10 (IP10). We developed and validated the assay and applied it to the detection of CMV, HIV and Mycobacterium tuberculosis (MTB) specific responses, in a cohort of HIV co-infected patients. We compared the sensitivity of this assay to that of the ex vivo RD1 (...

Expand abstract

Actions


Access Document


Authors


Journal:
PLoS ONE More from this journal
Volume:
6
Issue:
8
Publication date:
2011-01-01
DOI:
EISSN:
1932-6203
Language:
English
Pubs id:
pubs:179386
UUID:
uuid:0bb86dbd-a9fa-48cf-88d1-bcd79e5d0254
Local pid:
pubs:179386
Source identifiers:
179386
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP