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A lipid bound actin meshwork organizes liquid phase separation in model membranes

Abstract:

The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. A minimal actin cortex was bound to a supported lipid bilayer via biotinylated lipid streptavidin complexes (pinning sites). In general, actin binding to ternary membranes prevented macroscopic liquid-ordered and liquid-disordered domain formation, even at low temperature. Instead, dependin...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.7554/elife.01671

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Publisher:
eLife Sciences Publications Publisher's website
Journal:
eLife Journal website
Volume:
2014
Issue:
3
Article number:
e01671
Publication date:
2014-03-18
Acceptance date:
2014-02-14
DOI:
EISSN:
2050-084X
Language:
English
Keywords:
UUID:
uuid:0bb429a8-ca99-4b62-b8a8-320dbed2ae42
Local pid:
pubs:450621
Source identifiers:
450621
Deposit date:
2014-08-06

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