Identification and characterization of monoclonal antibodies specific for polymorphic antigenic determinants within the V2 region of the human immunodeficiency virus type 1 envelope glycoprotein.

Journal article

We have identified six monoclonal antibodies (MAbs) mapping to both linear and conformation-dependent epitopes within the V2 region of the human immunodeficiency virus type 1 clone HXB10. Three of the MAbs (12b, 66c, and 66a) were able to neutralize the molecular clones HXB10 and HXB2, with titers in the range of 9.5 to 20.0 micrograms/ml. MAbs mapping to the crown of the V2 loop (12b, 60b, and 74) bound poorly to cell surface-expressed oligomeric gp120, suggesting an explanation for the poor...

Expand abstract

Authors: Shotton, C; Arnold, C; Sattentau, Q; Sodroski, J; McKeating, J

• Publication status: Published
• Journal: Journal of virology
• Volume: 69
• Issue: 1
• Pages: 222-230
• Publication date: 1995-01-01
• EISSN: 1098-5514
• ISSN: 0022-538X
• Language: English
• Keywords: DNA Primers; Base Sequence; Cricetinae; Antibodies, Monoclonal; Epitopes; Amino Acid Sequence; Animals; Polymorphism, Genetic; Binding Sites, Antibody; HIV-1; CHO Cells; Molecular Sequence Data; HIV Envelope Protein gp120; Neutralization Tests