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Conformational changes induced by salt in complexes of histones and superhelical nuclear DNA.

Abstract:

When HeLa cells are lysed in solutions containing a non-ionic detergent and 0.75 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear DNA, RNA and the 'core' histones, but few other proteins characteristic of chromatin. Their DNA is intact. The core histones dissociate on raising the salt concentration. We have probed the structure of nucleoid-histone complexes using the intercalating dye, ethidium, or the RNA polyme...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell science
Volume:
50
Issue:
AUG
Pages:
199-208
Publication date:
1981-08-01
EISSN:
1477-9137
ISSN:
0021-9533
Language:
English
Keywords:
Pubs id:
pubs:21791
UUID:
uuid:0b76d6f0-8db6-4b41-a604-0bb5e0b28c40
Local pid:
pubs:21791
Source identifiers:
21791
Deposit date:
2012-12-19

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