Intraerythrocytic polyubiquitin expression in Plasmodium falciparum is subjected to developmental and heat-shock control.

Journal article

The polyubiquitin gene of the human protozoan parasite Plasmodium falciparum (PfpUB) was cloned and shown to be comprised of five tandem repeats of the ubiquitin open reading frame, present as a single copy on chromosome 12. The 1672 bp of PfpUB is interrupted at the 5' end by a single intron of 526 bp. PfpUB expression is developmentally regulated in intraerythrocytic stages with a marked increase in both steady-state transcript and polyubiquitin protein levels in late trophozoite stages. On...

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Authors: Horrocks, P; Newbold, C

• Publication status: Published
• Journal: Molecular and biochemical parasitology
• Volume: 105
• Issue: 1
• Pages: 115-125
• Publication date: 2000-01-01
• DOI: 10.1016/s0166-6851(99)00174-7
• EISSN: 1872-9428
• ISSN: 0166-6851
• Language: English
• Keywords: Biopolymers; Blotting, Northern; Sequence Analysis, DNA; Molecular Sequence Data; Humans; Open Reading Frames; Heat-Shock Response; Gene Expression Regulation; DNA, Protozoan; Polyubiquitin; Ubiquitins; Cloning, Molecular; Erythrocytes; Transcription, Genetic; Tandem Repeat Sequences; Amino Acid Sequence; Animals; Evolution, Molecular; Plasmodium falciparum