GATA3 is redundant for maintenance and self-renewal of hematopoietic stem cells.

Journal article

GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal.

Authors: Buza-Vidas, N; Duarte, S; Luc, S; Bouriez-Jones, T; Woll, P

• Publication status: Published
• Journal: Blood
• Volume: 118
• Issue: 5
• Pages: 1291-1293
• Publication date: 2011-08-01
• DOI: 10.1182/blood-2011-02-338046
• EISSN: 1528-0020
• ISSN: 0006-4971
• Language: English
• Keywords: Mice, Knockout; Mice, Inbred C57BL; Animals; Hematopoiesis; Hematopoietic Stem Cell Transplantation; Cell Proliferation; GATA3 Transcription Factor; Cells, Cultured; Hematopoietic Stem Cells; Mice; Signal Transduction