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Creating wounds in cell monolayers using micro-jets

Abstract:

Many wound-healing assays are used in cell biology and biomedicine; they are often labor intensive and/or require specialized and costly equipment. We describe a contactless method to create wounds with any imaginable 2D pattern in cell monolayers using the micro-jets of either media or an immiscible and biocompatible fluorocarbon (i.e., FC40). We also combine this with another method that allows automation and multiplexing using standard Petri dishes. A dish is filled with a thin film of med...

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Publication status:
Published
Peer review status:
Peer reviewed

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Role:
Author
ORCID:
0000-0003-3126-1708
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Institution:
University of Oxford
Role:
Author
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Role:
Author
ORCID:
0000-0001-5264-1561
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Name:
Royal Society
Grant:
URF/R/180016
RGF\EA\181002
Publisher:
AIP Publishing
Journal:
Biomicrofluidics More from this journal
Volume:
15
Issue:
1
Article number:
014108
Publication date:
2021-02-08
Acceptance date:
2021-01-26
DOI:
EISSN:
1932-1058
ISSN:
1932-1058
Pmid:
33598064
Language:
English
Keywords:
Pubs id:
1163890
Local pid:
pubs:1163890
Deposit date:
2021-07-21

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