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Post-translational mutagenesis for installation of natural and unnatural amino acid side chains into recombinant proteins

Abstract:

Methods for installing natural and unnatural amino acids and their modifications into proteins in a benign and precise manner are highly sought-after in protein science. Here we describe a protocol for 'post-translational mutagenesis' that enables the programmed installation of protein side chains through the use of rapid, mild and operationally simple free-radical chemistry performed on recombinantly expressed and purified proteins. By introduction of protein dehydroalanine (Dha) residues (i...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1038/nprot.2017.087

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
Oxford college:
Pembroke College
Role:
Author
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Funding agency for:
Wright, TH
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Funding agency for:
Davis, BG
Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Protocols Journal website
Volume:
12
Issue:
10
Pages:
2243-2250
Publication date:
2017-09-29
Acceptance date:
2017-05-30
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pubs id:
pubs:738672
URN:
uri:0ad1e386-ed45-4582-9b97-6ed03ca1f10a
UUID:
uuid:0ad1e386-ed45-4582-9b97-6ed03ca1f10a
Local pid:
pubs:738672
Paper number:
10

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