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Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis.

Abstract:

We report a single-molecule assay that defines, simultaneously, the translocational position of a protein complex relative to DNA and the subunit stoichiometry of the complex. We applied the assay to define translocational positions and sigma70 contents of bacterial transcription elongation complexes in vitro. The results confirm ensemble results indicating that a large fraction, approximately 70%-90%, of early elongation complexes retain sigma70 and that a determinant for sigma70 recognition...

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Journal:
Molecular cell
Volume:
20
Issue:
3
Pages:
347-356
Publication date:
2005-11-01
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
Source identifiers:
15323
Language:
English
Keywords:
Pubs id:
pubs:15323
UUID:
uuid:0ad10115-0ed8-4e28-be20-383d2d1e5cef
Local pid:
pubs:15323
Deposit date:
2012-12-20

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