Journal article
A comparative evaluation of the effect of polymer chemistry and fiber orientation on mesenchymal stem cell differentiation.
- Abstract:
- Bioengineered tissue scaffolds in combination with cells hold great promise for tissue regeneration. The aim of this study was to determine how the chemistry and fibre orientation of engineered scaffolds affect the differentiation of mesenchymal stem cells (MSCs). Adipogenic, chondrogenic and osteogenic differentiation on aligned and randomly orientated electrospun scaffolds of Poly (lactic-co-glycolic) acid (PLGA) and Polydioxanone (PDO) were compared. MSCs were seeded onto scaffolds and cultured for 14 days under adipogenic-, chondrogenic-or osteogenic-inducing conditions. Cell viability was assessed by alamarBlue metabolic activity assays and gene expression was determined by qRT-PCR. Cell-scaffold interactions were visualized using fluorescence and scanning electron microscopy. Cells grew in response to scaffold fibre orientation and cell viability, cell coverage and gene expression analysis showed that PDO supports greater multilineage differentiation of MSCs. An aligned PDO scaffold supports highest adipogenic and osteogenic differentiation whereas fibre orientation did not have a consistent effect on chondrogenesis. Electrospun scaffolds, selected on the basis of fibre chemistry and alignment parameters could provide great therapeutic potential for restoration of fat, cartilage and bone tissue. This study supports the continued investigation of an electrospun PDO scaffold for tissue repair and regeneration and highlights the potential of optimizing fibre orientation for improved utility.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 907.2KB, Terms of use)
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- Publisher copy:
- 10.1002/jbm.a.35829
Authors
- Publisher:
- Wiley
- Journal:
- Journal of Biomedical Materials Research. Part A More from this journal
- Volume:
- 104
- Issue:
- 11
- Pages:
- 2843-2853
- Publication date:
- 2016-07-01
- Acceptance date:
- 2016-06-27
- DOI:
- ISSN:
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1552-4965 and 1549-3296
- Pmid:
-
27399850
- Language:
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English
- Keywords:
- Pubs id:
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pubs:634075
- UUID:
-
uuid:0ad078c4-9855-4eba-8a3c-a2c9046ce66e
- Local pid:
-
pubs:634075
- Source identifiers:
-
634075
- Deposit date:
-
2016-11-25
Terms of use
- Copyright holder:
- Snelling
- Copyright date:
- 2016
- Notes:
- © 2016 The Authors. Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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