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Methodology employed for the structure determination of tumour necrosis factor, a case of high non-crystallographic symmetry.

Abstract:

The structure of the protein tumour necrosis factor (TNF) was determined from crystals of space group P3(1)21 which contain six copies of the TNF monomer per crystallographic asymmetric unit [Jones, Stuart and Walker (1989). Nature (London), 338, 225-228]. The nature of these crystals (relatively high crystallographic symmetry coupled with multiple copies of the protein in the asymmetric unit) led to some peculiarly challenging problems at several points in the structure determination. In par...

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Publisher copy:
10.1107/S0108767391006839

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Acta Crystallographica Section A: Foundations of Crystallography
Volume:
47
Publication date:
1991-11-01
DOI:
ISSN:
0108-7673
Language:
English
Pubs id:
pubs:94064
UUID:
uuid:0a82feb6-dba3-44a2-9499-700092e6a3c8
Local pid:
pubs:94064
Source identifiers:
94064
Deposit date:
2013-02-20

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