Journal article

rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.

Abstract:: We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Kwok, C., Marsico, G., Sahakyan, A., Chambers, V., & Balasubramanian, S. (2016). rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. Nature Methods, 13(10), 841–844.

MLA Style

Kwok, C., et al. “rG4-Seq Reveals Widespread Formation of G-Quadruplex Structures in the Human Transcriptome.” Nature Methods, vol. 13, no. 10, Nature Publishing Group, 2016, pp. 841–44.

Chicago Style

Kwok, C, G Marsico, A Sahakyan, V Chambers, and S Balasubramanian. 2016. “rG4-Seq Reveals Widespread Formation of G-Quadruplex Structures in the Human Transcriptome.” Nature Methods 13 (10): 841–44.
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Files:: Sahakyan et al, rG4-seq reveals widespread formation of G-quad...

(Preview, Accepted manuscript, pdf, 13.8MB, Terms of use)

Publisher copy:: 10.1038/nmeth.3965

Authors

+ Kwok, C More by this author

Role:: Author

+ Marsico, G More by this author

Role:: Author

+ Sahakyan, A More by this author

Institution:: University of Oxford
Division:: Medical Sciences Division
Department:: RDM, RDM Clinical Laboratory Sciences
Role:: Author

+ Chambers, V More by this author

Role:: Author

+ Balasubramanian, S More by this author

Role:: Author

+ Wellcome Trust More from this funder

Grant:: 099232/z/12/z

+ Biotechnology and Biological Sciences Research Council More from this funder

Grant:: BB/I015477/1

+ European Research Council More from this funder

Grant:: 339778

+ Croucher Foundation More from this funder

+ Herchel Smith Fellowship More from this funder

Publisher:: Nature Publishing Group
Journal:: Nature Methods More from this journal
Volume:: 13
Issue:: 10
Pages:: 841-844
Publication date:: 2016-08-01
Acceptance date:: 2016-07-21
DOI:: 10.1038/nmeth.3965
EISSN:: 1548-7105
ISSN:: 1548-7091
Pmid:: 27571552

Language:: English
Keywords:: Humans

Transcriptome

RNA Stability

G-Quadruplexes

Guanine

RNA, Messenger

Cytosine

Sequence Analysis, RNA

High-Throughput Nucleotide Sequencing

Hela Cells
Pubs id:: pubs:729660
UUID:: uuid:0a7ec504-3650-4c6b-831f-73ccffdf60a4
Local pid:: pubs:729660
Source identifiers:: 729660
Deposit date:: 2017-11-10

Terms of use

Copyright holder:: Nature America, Inc
Copyright date:: 2016
Notes:: © 2016 Nature America, Inc. All rights reserved

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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