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rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.

Abstract:

We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1038/nmeth.3965

Authors


Marsico, G More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM, RDM Clinical Laboratory Sciences
Chambers, VS More by this author
Balasubramanian, S More by this author
Herchel Smith Fellowship More from this funder
Croucher Foundation More from this funder
Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Methods Journal website
Volume:
13
Issue:
10
Pages:
841-844
Publication date:
2016-08-05
Acceptance date:
2016-07-21
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pubs id:
pubs:729660
URN:
uri:0a7ec504-3650-4c6b-831f-73ccffdf60a4
UUID:
uuid:0a7ec504-3650-4c6b-831f-73ccffdf60a4
Local pid:
pubs:729660

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