Journal article
rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.
- Abstract:
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We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding ...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted manuscript
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(pdf, 13.8MB)
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- Publisher copy:
- 10.1038/nmeth.3965
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Authors
Funding
Herchel Smith Fellowship
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Croucher Foundation
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Bibliographic Details
- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Nature Methods Journal website
- Volume:
- 13
- Issue:
- 10
- Pages:
- 841-844
- Publication date:
- 2016-08-05
- Acceptance date:
- 2016-07-21
- DOI:
- EISSN:
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1548-7105
- ISSN:
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1548-7091
- Pubs id:
-
pubs:729660
- URN:
-
uri:0a7ec504-3650-4c6b-831f-73ccffdf60a4
- UUID:
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uuid:0a7ec504-3650-4c6b-831f-73ccffdf60a4
- Local pid:
- pubs:729660
Item Description
- Language:
- English
- Keywords:
Terms of use
- Copyright holder:
- Nature America, Inc
- Copyright date:
- 2016
- Notes:
- © 2016 Nature America, Inc. All rights reserved
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