Granulocyte-colony stimulating factor induced intranuclear endonuclease in murine leukemia cell line.

Journal article

We have reported that murine leukemia cell line (C2M-A5) induced apoptosis by G-CSF. To clarify the mechanism, mRNA expression of apoptosis-related genes was studied. It revealed transient over-expression of c-myc, H-ras and p53 and down-expression of bcl-2. These changes were known as triggers of endonuclease induction. After 96 h culture with G-CSF, apoptosis was occurred simultaneously with endonuclease (37 kd) activation. This endonuclease induced the digestion of double-strand DNA and might be associated with caspase3. Although G-CSF accelerates cell growth and prevents apoptosis in general, it is a contradictory effect. We concluded that G-CSF induced endogenous endonuclease activity in C2M-A5.

Authors: Handa, A; Kashimura, T; Yamamoto, A; Murohashi, I; Bessho, M

• Publication status: Published
• Journal: Leukemia research
• Volume: 24
• Issue: 12
• Pages: 1033-1039
• Publication date: 2000-12-01
• DOI: 10.1016/s0145-2126(00)00074-6
• EISSN: 1873-5835
• ISSN: 0145-2126
• Language: English
• Keywords: Mice; Caspase 3; Endonucleases; Genes, bcl-2; Gene Expression; Tumor Cells, Cultured; Caspases; Granulocyte Colony-Stimulating Factor; Animals; Genes, myc; Apoptosis; RNA, Messenger; Genes, p53; Leukemia, Myeloid; Genes, ras