Journal article icon

Journal article

A single subunit directs the assembly of the Escherichia coli DNA sliding clamp loader.

Abstract:

Multi-protein clamp loader complexes are required to load sliding clamps onto DNA. In Escherichia coli the clamp loader contains three DnaX (tau/gamma) proteins, delta, and delta', which together form an asymmetric pentameric ring that also interacts with psichi. Here we used mass spectrometry to examine the assembly and dynamics of the clamp loader complex. We find that gamma exists exclusively as a stable homotetramer, while tau is in a monomer-dimer-trimer-tetramer equilibrium. delta' play...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1016/j.str.2010.01.009

Authors


Journal:
Structure (London, England : 1993) More from this journal
Volume:
18
Issue:
3
Pages:
285-292
Publication date:
2010-03-01
DOI:
EISSN:
1878-4186
ISSN:
0969-2126
Language:
English
Keywords:
Pubs id:
pubs:59336
UUID:
uuid:0a69dbc2-5efd-4a54-b051-c632d8d9f827
Local pid:
pubs:59336
Source identifiers:
59336
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP