A single subunit directs the assembly of the Escherichia coli DNA sliding clamp loader.

Journal article

Multi-protein clamp loader complexes are required to load sliding clamps onto DNA. In Escherichia coli the clamp loader contains three DnaX (tau/gamma) proteins, delta, and delta', which together form an asymmetric pentameric ring that also interacts with psichi. Here we used mass spectrometry to examine the assembly and dynamics of the clamp loader complex. We find that gamma exists exclusively as a stable homotetramer, while tau is in a monomer-dimer-trimer-tetramer equilibrium. delta' play...

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Authors: Park, A; Jergic, S; Politis, A; Ruotolo, B; Hirshberg, D

• Publication status: Published
• Journal: Structure (London, England : 1993)
• Volume: 18
• Issue: 3
• Pages: 285-292
• Publication date: 2010-03-01
• DOI: 10.1016/j.str.2010.01.009
• EISSN: 1878-4186
• ISSN: 0969-2126
• Language: English
• Keywords: DNA, Bacterial; Escherichia coli Proteins; Bacterial Proteins; Escherichia coli; Protein Subunits; Models, Biological; Binding Sites; DNA Polymerase III