Identification of Tyr58 as the proton donor in the aspartate-alpha-decarboxylase reaction.

Journal article

The decarboxylation of L-aspartate by E. coli L-aspartate-alpha-decarboxylase (ADC) is shown to occur with retention of configuration; analysis of the protein structure identifies Tyr58 as the proton donor in the decarboxylation mechanism.

Authors: Saldanha, SA; Birch, L; Webb, M; Nabbs, B; von Delft, F

• Publication status: Published
• Journal: Chemical Communications
• Issue: 18
• Pages: 1760-1761
• Publication date: 2001-09-01
• DOI: 10.1039/b106090m
• EISSN: 1364-548X
• ISSN: 1359-7345
• Language: English
• Keywords: Glutamate Decarboxylase; Gene Expression Regulation, Bacterial; Protons; Nuclear Magnetic Resonance, Biomolecular; Models, Molecular; Binding Sites; Tyrosine; Protein Conformation; Escherichia coli; Stereoisomerism