Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae.

Journal article

Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.

Authors: Akiyoshi, B; Nelson, C; Ranish, J; Biggins, S

• Journal: Genetics
• Volume: 183
• Issue: 4
• Pages: 1591-1595
• Publication date: 2009-12-01
• DOI: 10.1534/genetics.109.109041
• EISSN: 1943-2631
• ISSN: 0016-6731
• Language: English
• Keywords: Amino Acid Sequence; Saccharomyces cerevisiae Proteins; Mutation; Intracellular Signaling Peptides and Proteins; Nuclear Proteins; Kinetochores; Phosphorylation; Saccharomyces cerevisiae; Protein-Serine-Threonine Kinases; Microtubules