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Detection and selective dissociation of intact ribosomes in a mass spectrometer.

Abstract:

Intact Escherichia coli ribosomes have been projected into the gas phase of a mass spectrometer by means of nanoflow electrospray techniques. Species with mass/charge ratios in excess of 20,000 were detected at the level of individual ions by using time-of-flight analysis. Once in the gas phase the stability of intact ribosomes was investigated and found to increase as a result of cross-linking ribosomal proteins to the rRNA. By lowering the Mg(2+) concentration in solutions containing riboso...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.97.10.5185

Authors


Rostom, AA More by this author
Benjamin, DR More by this author
Juenemann, R More by this author
Nierhaus, KH More by this author
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Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
97
Issue:
10
Pages:
5185-5190
Publication date:
2000-05-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:0a2f7356-2e22-440c-bb2d-3ab08eb25f9a
Source identifiers:
59227
Local pid:
pubs:59227

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