- Abstract:
-
Intact Escherichia coli ribosomes have been projected into the gas phase of a mass spectrometer by means of nanoflow electrospray techniques. Species with mass/charge ratios in excess of 20,000 were detected at the level of individual ions by using time-of-flight analysis. Once in the gas phase the stability of intact ribosomes was investigated and found to increase as a result of cross-linking ribosomal proteins to the rRNA. By lowering the Mg(2+) concentration in solutions containing riboso...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1073/pnas.97.10.5185
-
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- Journal:
- Proceedings of the National Academy of Sciences of the United States of America
- Volume:
- 97
- Issue:
- 10
- Pages:
- 5185-5190
- Publication date:
- 2000-05-05
- DOI:
- EISSN:
-
1091-6490
- ISSN:
-
0027-8424
- URN:
-
uuid:0a2f7356-2e22-440c-bb2d-3ab08eb25f9a
- Source identifiers:
-
59227
- Local pid:
- pubs:59227
- Language:
- English
- Keywords:
- Copyright date:
- 2000
Journal article
Detection and selective dissociation of intact ribosomes in a mass spectrometer.
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