The acidic macromolecules in rabbit cortical bone tissue.

Journal article

Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.

Authors: Diamond, A; Triffitt, J; Herring, G

• Publication status: Published
• Journal: Archives of oral biology
• Volume: 27
• Issue: 4
• Pages: 337-345
• Publication date: 1982-01-01
• DOI: 10.1016/0003-9969(82)90164-9
• EISSN: 1879-1506
• ISSN: 0003-9969
• Language: English
• Keywords: Macromolecular Substances; Electrophoresis, Polyacrylamide Gel; Sialoglycoproteins; Chondroitin Sulfates; Edetic Acid; Glycosaminoglycans; Keratan Sulfate; Chromatography; Proteoglycans; Animals; Bone and Bones; Rabbits