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Journal article

Single-molecule imaging of DNA curtains reveals mechanisms of KOPS sequence targeting by the DNA translocase FtsK.

Abstract:

FtsK is a hexameric DNA translocase that participates in the final stages of bacterial chromosome segregation. Here we investigate the DNA-binding and translocation activities of FtsK in real time by imaging fluorescently tagged proteins on nanofabricated curtains of DNA. We show that FtsK preferentially loads at 8-bp KOPS (FtsK Orienting Polar Sequences) sites and that loading is enhanced in the presence of ADP. We also demonstrate that FtsK locates KOPS through a mechanism that does not inv...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.1201613109

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
109
Issue:
17
Pages:
6531-6536
Publication date:
2012-04-09
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Source identifiers:
322712
Language:
English
Keywords:
Pubs id:
pubs:322712
UUID:
uuid:09fc127b-5b7d-4d5e-9ace-08601f1bf262
Local pid:
pubs:322712
Deposit date:
2013-11-17

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