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High throughput measurement of duplex, triplex and quadruplex melting curves using molecular beacons and a LightCycler.

Abstract:

We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used in these studies contain a fluorophore (fluorescein) and quencher (methyl red) attached either to deoxyribose or to the 5 position of dU. In the folded DNA structures the fluorophore and quencher are in close proximity and the fluorescence is quenched. When the structures melt, the fluorophore an...

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Publication status:
Published

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Publisher copy:
10.1093/nar/30.9.e39

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Journal:
Nucleic acids research More from this journal
Volume:
30
Issue:
9
Pages:
e39
Publication date:
2002-05-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Keywords:
Pubs id:
pubs:400062
UUID:
uuid:09ed7fc6-3d78-4046-9ffb-2b51546d58d4
Local pid:
pubs:400062
Source identifiers:
400062
Deposit date:
2013-11-16

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