The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage.

Journal article

The Saccharomyces cerevisiae RAD9 checkpoint gene is required for transient cell-cycle arrests and transcriptional induction of DNA repair genes in response to DNA damage. Polyclonal antibodies raised against the Rad9 protein recognized several polypeptides in asynchronous cultures, and in cells arrested in S or G2/M phases while a single form was observed in G1-arrested cells. Treatment with various DNA damaging agents, i.e. UV, ionizing radiation or methyl methane sulfonate, resulted in the...

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Authors: Vialard, J; Gilbert, C; Green, C; Lowndes, N

• Publication status: Published
• Journal: EMBO journal
• Volume: 17
• Issue: 19
• Pages: 5679-5688
• Publication date: 1998-10-01
• DOI: 10.1093/emboj/17.19.5679
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Methyl Methanesulfonate; Phosphorylation; Protein Kinases; Hydroxyurea; Mutagens; G2 Phase; Mutation; Protein-Serine-Threonine Kinases; Intracellular Signaling Peptides and Proteins; Saccharomyces cerevisiae Proteins; Cell Cycle Proteins; Ultraviolet Rays; Mitosis; Fungal Proteins; DNA Damage; Protein Binding; Saccharomyces cerevisiae; Gamma Rays; S Phase; Nocodazole