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The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage.

Abstract:

The Saccharomyces cerevisiae RAD9 checkpoint gene is required for transient cell-cycle arrests and transcriptional induction of DNA repair genes in response to DNA damage. Polyclonal antibodies raised against the Rad9 protein recognized several polypeptides in asynchronous cultures, and in cells arrested in S or G2/M phases while a single form was observed in G1-arrested cells. Treatment with various DNA damaging agents, i.e. UV, ionizing radiation or methyl methane sulfonate, resulted in the...

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Publication status:
Published

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Publisher copy:
10.1093/emboj/17.19.5679
Journal:
EMBO journal More from this journal
Volume:
17
Issue:
19
Pages:
5679-5688
Publication date:
1998-10-01
DOI:
EISSN:
1460-2075
ISSN:
0261-4189

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