Journal article
An immunocytochemical assay to detect human CFTR expression following gene transfer.
- Abstract:
-
BACKGROUND: To assess gene therapy treatment for cystic fibrosis (CF) in clinical trials it is essential to develop robust assays that can accurately detect transgene expression in human airway epithelial cells. Our aim was to develop a reproducible immunocytochemical assay for human CFTR protein which can measure both endogenous CFTR levels and augmented CFTR expression after gene delivery. METHODS: We characterised an antibody (G449) which satisfied the criteria for use in clinical trials....
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- Publication status:
- Published
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- Publisher copy:
- 10.1016/j.mcp.2009.07.001
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Bibliographic Details
- Journal:
- Molecular and cellular probes
- Volume:
- 23
- Issue:
- 6
- Pages:
- 272-280
- Publication date:
- 2009-12-01
- DOI:
- EISSN:
-
1096-1194
- ISSN:
-
0890-8508
- Source identifiers:
-
116564
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:116564
- UUID:
-
uuid:09e25980-c034-4ff9-8f96-841ff1e8965f
- Local pid:
- pubs:116564
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2009
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