Journal article icon

Journal article

Detergent-free incorporation of a seven-transmembrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies.

Abstract:
SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1021/nl3020395

Authors


Expand authors...
Journal:
Nano letters
Volume:
12
Issue:
9
Pages:
4687-4692
Publication date:
2012-09-05
DOI:
EISSN:
1530-6992
ISSN:
1530-6984
URN:
uuid:09c633e9-feb4-4aef-b251-998032b08b68
Source identifiers:
344175
Local pid:
pubs:344175

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP