Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages.

Mahajan, L; Madan, T; Kamal, N; Singh, V; Sim, R; Telang, S; Ramchand, C; Waters, P; Kishore, U; Sarma, P

Journal article

Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages.

Abstract:: Pulmonary surfactant protein-D (SP-D) is a multifunctional, pattern recognition molecule involved in resistance to allergen challenge and pulmonary inflammation. In view of therapeutic effects of exogenous SP-D or recombinant fragment of human surfactant protein-D (rhSP-D) (composed of eight Gly-X-Y collagen repeat sequences, homotrimeric neck and lectin domains) in murine models of lung allergy and hypereosinophilic SP-D gene-deficient mice, we investigated the possibility of a direct interaction of purified rhSP-D with human eosinophils derived from allergic patients and healthy donors. rhSP-D showed a sugar- and calcium-dependent binding to human eosinophils, suggesting involvement of its carbohydrate recognition domain. While eosinophils from allergic patients showed a significant increase in apoptosis, oxidative burst and CD69 expression in presence of rhSP-D, eosinophils from healthy donors showed no significant change. However, these eosinophils from healthy donors when primed with IL-5 exhibited increase in apoptosis on incubation with rhSP-D. Apoptosis mediated by rhSP-D in primed eosinophils was not affected by the antioxidant, N-acetyl-L-cysteine. There was a manifold increase in binding of rhSP-D to apoptotic eosinophils than the normal eosinophils and rhSP-D induced a significant increase in uptake of apoptotic eosinophils by J774A.1 macrophage cells. The study suggests that rhSP-D mediated preferential increase of apoptosis of primed eosinophils while not affecting the normal eosinophils and increased phagocytosis of apoptotic eosinophils may be important mechanisms of rhSP-D and plausibly SP-D-mediated resolution of allergic eosinophilic inflammation in vivo.

Publication status:: Published

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Share
Cite

Cite this record

APA Style

Mahajan, L., Madan, T., Kamal, N., Singh, V., Sim, R., Telang, S., Ramchand, C., Waters, P., Kishore, U., & Sarma, P. (2008). Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages. International Immunology, 20(8), 993–1007.

MLA Style

Mahajan, L, et al. “Recombinant Surfactant Protein-D Selectively Increases Apoptosis in Eosinophils of Allergic Asthmatics and Enhances Uptake of Apoptotic Eosinophils by Macrophages.” International Immunology, vol. 20, no. 8, 2008, pp. 993–1007.

Chicago Style

Mahajan, L, T Madan, N Kamal, et al. 2008. “Recombinant Surfactant Protein-D Selectively Increases Apoptosis in Eosinophils of Allergic Asthmatics and Enhances Uptake of Apoptotic Eosinophils by Macrophages.” International Immunology 20 (8): 993–1007.
Print