Optimization of in vivo confocal autofluorescence imaging of the ocular fundus in mice and its application to models of human retinal degeneration.

Journal article

PURPOSE: To investigate the feasibility and to identify sources of experimental variability of quantitative and qualitative fundus autofluorescence (AF) assessment in mice. METHODS: Blue (488 nm) and near-infrared (790 nm) fundus AF imaging was performed in various mouse strains and disease models (129S2, C57Bl/6, Abca4(-/-), C3H-Pde6b(rd1/rd1), Rho(-/-), and BALB/c mice) using a commercially available scanning laser ophthalmoscope. Gray-level analysis was used to explore factors influencing...

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Authors: Charbel Issa, P; Singh, MS; Lipinski, D; Chong, V; Delori, F

• Publication status: Published
• Journal: Investigative ophthalmology and visual science
• Volume: 53
• Issue: 2
• Pages: 1066-1075
• Publication date: 2012-02-29
• DOI: 10.1167/iovs.11-8767
• EISSN: 1552-5783
• ISSN: 0146-0404
• Language: English
• Keywords: Fundus Oculi; Fluorescence; Mice, Inbred C57BL; Mice, Inbred BALB C; Disease Models, Animal; Animals; Diagnostic Techniques, Ophthalmological; Mice; Microscopy, Confocal; Feasibility Studies; Mice, Inbred C3H; Reproducibility of Results; Pupil; Retinal Degeneration