Reversal of the effects of a low extracellular potassium concentration on the number and activity of Na+/K+ pumps in an Epstein-Barr virus-transformed human lymphocyte cell line.

Journal article

A reduction in the extracellular concentration of potassium to 0.5 mM (low K) in Epstein-Barr (EB) virus-transformed lymphocytes caused changes in the number and activity of Na+/K+ pumps in the cell membrane, with increases in the Bmax and apparent Kd of ouabain binding, and concomitant increases in the Vmax and apparent Km of potassium (rubidium) influx. However, recovery from the effects of low K occurred more quickly than the original up-regulation. Furthermore, there were differences in t...

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Authors: Ameen, M; Bloomfield, J; Aronson, J

• Publication status: Published
• Journal: Biochemical pharmacology
• Volume: 43
• Issue: 3
• Pages: 489-496
• Publication date: 1992-02-01
• DOI: 10.1016/0006-2952(92)90568-4
• EISSN: 1873-2968
• ISSN: 0006-2952
• Language: English
• Keywords: Up-Regulation; Kinetics; Ouabain; Lymphocytes; Rubidium; Cell Line, Transformed; Protein Biosynthesis; Humans; Herpesvirus 4, Human; Sodium-Potassium-Exchanging ATPase; Potassium